METAGENOMICS: DNA SEQUENCING OF UNCULTURED MICROORGANISMS

Microbiology has completely transformed in the last 25 years and realization that most microorganisms cannot be grown a pure culture resulted new methods of analyzing genomic diversity. One such method, Metagenomics, refers to study on basis their diversity, acquired directly from environmental samples. It facilitates physiology ecology through vast array sequencing functional techniques. Over years, many techniques like Sangers Sequencing, Shotgun Whole Metagenomic Sequencing 16s rRNA gene were used, which revolutionized portrait microbial world. Microbiome profiles these samples can generated analysed various statistical tools annotations, marker data profiling biomarker detection give description community membership, provide insight into genetics biochemistry members. The application metagenomic sequence information will result better culturing strategies help link analysis with studies.